Uuendan väheke andmeid. Seni on maailm arvanud, et 60 kraadi umbes 30 minuti juures peaks piisama viiruse äraküpsemiseks. Hetkel on välja tulnud uus uurimus, mis siiski soovitab 92 kraadi ja 15 minutit ja uuringu autorite järgi on see efektiivsem kui 60 kraadi ja 60 minutit!Tux kirjutas:Kuna asi on huvitav ja ametlikust meediast on mulle vähe, siis olen otsinud omal käel väheke lisamaterjali, mida tahan teiega jagada. Järsku on huvitav ja kasulik! Enamik viidatud publikatsioone on vabalt kõigile kättesaadavad.
Koroonaviirusest endast veidi
Stability and inactivation of SARS coronavirus
https://www.ncbi.nlm.nih.gov/pubmed/15118911Inactivation of SARS coronavirus by means of povidone-iodine, physical conditions, and chemical reagents.While direct person-to-person transmission via respiratory droplets accounted for most cases, other modes have not been ruled out. Faecal shedding is common and prolonged and has caused an outbreak in Hong Kong. We studied the stability of SARS-CoV under different conditions, both in suspension and dried on surfaces, in comparison with other human-pathogenic viruses, including human coronavirus HCoV-229E. In suspension, HCoV-229E gradually lost its infectivity completely while SARS-CoV retained its infectivity for up to 9 days; in the dried state, survival times were 24 h versus 6 days. Thermal inactivation at 56 degrees C was highly effective in the absence of protein, reducing the virus titre to below detectability; however, the addition of 20% protein exerted a protective effect resulting in residual infectivity. If protein-containing solutions are to be inactivated, heat treatment at 60 degrees C for at least 30 min must be used.
https://www.ncbi.nlm.nih.gov/pubmed/15631008Human Coronaviruses: Insights into Environmental Resistance and Its Influence on the Development of New Antiseptic StrategiesThe efficacy of several povidone-iodine (PVP-I) products, a number of other chemical agents, and various physical conditions were evaluated for their ability to inactivate the severe acute respiratory syndrome coronavirus (SARS-CoV). Treatment of SARS-CoV with PVP-I products for 2 min reduced the virus infectivity from 1.17 x 10(6) TCID50/ml to below the detectable level. The efficacy of 70% ethanol was equivalent to that of PVP-I products. Fixation of SARS-CoV-infected Vero E6 cells with a fixative including formalin, glutaraldehyde, methanol, and acetone for 5 min or longer eliminated all infectivity. Heating the virus at 56 degrees C for 5 min dramatically reduced the infectivity of the virus from 2.6 x 10(7) to 40 TCID50/ml, whereas heating the virus for 60 min or longer eliminated all infectivity. Irradiation with ultraviolet light at 134 microW/cm2 for 15 min reduced the infectivity from 3.8 x 10(7) to 180 TCID50/ml; however, prolonged irradiation (60 min) failed to eliminate the remaining virus, leaving 18.8 TCID50/ml.
https://www.ncbi.nlm.nih.gov/pubmed/23202515
Siin on 25 lehekülge väga selgeid juhendeid selle kohta, mis aitab seda viirust hävitada ja mis mitte!Temperature-dependent innate defense against the common cold virus limits viral replication at warm temperature in mouse airway cells.Thus, at 20 °C, aerosolized HCoV 229E was found to better survive at 50% relative humidity than at 30%. Indeed, nearly 20% of the original infectious virus was still detectable after six days. High relative humidity seemed less favorable to the virus, unless the temperature came down to 6 °C. At this temperature, the survival of the HCoV 229E was significantly enhanced whatever the rate of relative humidity. This enhanced survival rate at high relative humidity and low temperature may explain the winter propagation of coronaviruses.
Moreover, the HCoV 229E survival was significantly higher at 30% and 50% of relative humidity than those of the poliovirus in the same experimental conditions, which could be a striking result according to its nonenveloped nature [104].
Sensitivity of SARS-CoV to temperature has also been assayed. The exposure of the virus to a temperature of 56 °C over 30 min reduced virus titer under an undetectable level, except if SARS-CoV is associated with proteins, such as 20% fetal calf serum (FCS), which bring a protection for the virus. In this case, the temperature needs to reach 60 °C over 30 min to bring virus titer below the detection limit. This emphasizes the importance of organic material in which viruses could be embedded in the real conditions and could protect the virus, mostly from disinfection procedures. When the virus was placed at 4 °C, there was no loss of infectivity [105].
Another study confirms the viral stability at 4 °C, and also at 20 °C and 37 °C for at least 2 hrs, but SARS-CoV lost its infectivity after 90, 60 and 30 min exposure at 56 °C, 67 °C and 75 °C, respectively
https://www.ncbi.nlm.nih.gov/pubmed/25561542https://www.ncbi.nlm.nih.gov/pubmed/14631830Most isolates of human rhinovirus, the common cold virus, replicate more robustly at the cool temperatures found in the nasal cavity (33-35 °C) than at core body temperature (37 °C). To gain insight into the mechanism of temperature-dependent growth, we compared the transcriptional response of primary mouse airway epithelial cells infected with rhinovirus at 33 °C vs. 37 °C. Mouse airway cells infected with mouse-adapted rhinovirus 1B exhibited a striking enrichment in expression of antiviral defense response genes at 37 °C relative to 33 °C, which correlated with significantly higher expression levels of type I and type III IFN genes and IFN-stimulated genes (ISGs) at 37 °C. Temperature-dependent IFN induction in response to rhinovirus was dependent on the MAVS protein, a key signaling adaptor of the RIG-I-like receptors (RLRs). Stimulation of primary airway cells with the synthetic RLR ligand poly I:C led to greater IFN induction at 37 °C relative to 33 °C at early time points poststimulation and to a sustained increase in the induction of ISGs at 37 °C relative to 33 °C. Recombinant type I IFN also stimulated more robust induction of ISGs at 37 °C than at 33 °C. Genetic deficiency of MAVS or the type I IFN receptor in infected airway cells permitted higher levels of viral replication, particularly at 37 °C, and partially rescued the temperature-dependent growth phenotype. These findings demonstrate that in mouse airway cells, rhinovirus replicates preferentially at nasal cavity temperature due, in part, to a less efficient antiviral defense response of infected cells at cool temperature.
Stability of SARS coronavirus in human specimens and environment and its sensitivity to heating and UV irradiationÜhesõnaga saab alkoholiga pritsides pindu töödelda, viirusele ei meeldi kõrgemad temperatuurid - umbes pool tundi 60 kraadi juures lagundab selle viiruse. Seega kui teil on kodus saun, siis kuumutage oma üleriideid, hingake kuuma auru ja nii saab desinfitseerida ka maske!! Kui tulete maskiga väljast, siis kuumutage seda 60-70 kraadi juures!RESULTS:
The results showed that SARS coronavirus in the testing condition could survive in serum, 1:20 diluted sputum and feces for at least 96 h, whereas it could remain alive in urine for at least 72 h with a low level of infectivity. The survival abilities on the surfaces of eight different materials and in water were quite comparable, revealing reduction of infectivity after 72 to 96 h exposure. Viruses stayed stable at 4 degrees C, at room temperature (20 degrees C) and at 37 degrees C for at least 2 h without remarkable change in the infectious ability in cells, but were converted to be non-infectious after 90-, 60- and 30-min exposure at 56 degrees C, at 67 degrees C and at 75 degrees C, respectively. Irradiation of UV for 60 min on the virus in culture medium resulted in the destruction of viral infectivity at an undetectable level.
Enda kuumutamine on ka sellepärast hea mõte, sest viirus teeb esimesena pesa nina- ja põsekoobastesse, kuna need puutuvad välisõhuga kokku ja seal on temperatuur madalam, kui mujal kehas j viirus on ju temperatuuritundlik! Kuuma auru sissehingamine võiks seega takistada viiruse edasist levikut kehas.
Pdf fail Evaluation of heating and chemical protocols for inactivating SARS-CoV-2
https://www.biorxiv.org/content/10.1101 ... 1.full.pdf
Olge terved!
